8.01 Determination of neutral detergent fibre (NDF)

8.1    Determination of neutral detergent fibre (NDF)

Apparatus

1)        Refluxing apparatus:  Tall pyrex  or  corning  beakers (spout less) of  about  500 ml capacity round bottom flask as condenser

2)        Sintered glass crucibles with coarse porosity (Grade 1) of about 50 ml capacity

3)        Electronic balance

4)        Vacuum pump

5)        Hot plate

6)        Wash bottle

7)        Hot air oven and muffle furnace

Reagents

 

 

Neutral detergent solution:

 

 

Distilled water

1 litre

 

Sodium lauryl sulphate

30 g

 

Disodium ethylene di-aminetetraacetate (EDTA) dehydrate

18.61 g

 

Sodium borate decahydrate

6.81 g

 

Disodium hydrogen phosphate (anhydrous)

4.56 g

 

2-ethoxyethanol (ethylene glycol monoethyl ether)

10 ml